To solve this problem, we need to understand the direction of RNA synthesis and the direction of reading the template DNA strand.$\newline \bullet$RNA synthesis occurs in the 5' to 3' direction. This means that new nucleotides are added to the 3' end of the growing RNA strand.$\newline \bullet$The template DNA strand is read in the 3' to 5' direction. This is because RNA polymerase moves along the template strand in this direction to synthesize RNA.$\newline$Therefore, the correct option is:$\newline$Direction of RNA synthesis: 5' \rightarrow 3'$\newline$Direction of reading of the template DNA strand: 3' \rightarrow 5'$\newline$This corresponds to Option 1.