Which of the following is not a feature of plasmids?

The taq polymerase enzyme is obtained from

Which of the following is a restriction endonuclease?

A gene whose expression helps to identify transformed cell is known as

The correct order of steps in Polymerase Chain Reaction (PCR) is

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

The specific palindromic sequence which is recognized by EcoRI is:

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

The sequence that controls the copy number of the linked DNA in the vector, is termed:

Match the organism with its use in biotechnology.

Choose the correct pair from the following:

Identify the wrong statement with regard to Restriction Enzymes.

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Plasmid pBR322 has PstI restriction enzyme site within gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactosidase production and the recombinant plasmid is introduced in an $E. coli$ strain:

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). $\newline$ Assertion (A) : $\newline$ Polymerase chain reaction is used in DNA amplification. $\newline$ Reason (R) : $\newline$ The ampicillin resistant gene is used as a selectable marker to check transformation. $\newline$ In the light of the above statements, choose the correct answer from the options given below:

$\newline$Given below are two statements: $\newline$ $\newline$Statement I: Restriction endonucleases recognize specific sequences to cut DNA known as palindromic nucleotide sequences. $\newline$Statement II: Restriction endonucleases cut the DNA strand a little away from the center of the palindromic site. $\newline$ $\newline$In the light of the above statements, choose the most appropriate answer from the options given below:

In gene gun method used to introduce alien DNA into host cells, microparticles of _______ metal are used.

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence: $\newline$A. Insertion of recombinant DNA into the host cell. $\newline$B. Cutting of DNA at specific location by restriction enzyme. $\newline$C. Isolation of desired DNA fragment. $\newline$D. Amplification of gene of interest using PCR. $\newline$Choose the correct answer from the options given below:$$

Which of the following is not a cloning vector?

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? $\newline$ A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. $\newline$ B. It may get integrated into the genome of the recipient. $\newline$ C. It may multiply and be inherited along with the host DNA. $\newline$ D. The alien piece of DNA is not an integral part of chromosome. $\newline$ E. It shows ability to replicate.